Hotspot

atpB-rbcL

Genome · 57319-58072 · 754 bp

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Pi 0.00339

MarkerSeek score 65.40

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: Genome
length_bp: 754
variable_sites: 7
pi: 0.00339
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 65.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	TACCGGGCCAATGATTTGAG	GCCTTGAATCCAACACTTGC	50.0	50.0	57.9	57.9	864-895 bp	1.0	82.1
atpB-rbcL_p2	GGGGGAAAAGCTACATCCAG	GCCTTGAATCCAACACTTGC	55.0	50.0	57.9	57.9	884-915 bp	1.0	82.0
atpB-rbcL_p3	TACCGGGCCAATGATTTGAG	CCAGCCTTGAATCCAACACT	50.0	50.0	57.9	58.1	867-898 bp	1.0	82.0
atpB-rbcL_p4	GGGGGAAAAGCTACATCCAG	CCAGCCTTGAATCCAACACT	55.0	50.0	57.9	58.1	887-918 bp	1.0	81.9
atpB-rbcL_p5	ATACGCCCCAGGTTTCTTTC	GCCTTGAATCCAACACTTGC	50.0	50.0	57.9	57.9	842-873 bp	1.0	81.6