Hotspot

petN-psbM

Genome · 30871-31883 · 1013 bp

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Pi 0.00244

MarkerSeek score 60.50

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: Genome
length_bp: 1013
variable_sites: 8
pi: 0.00244
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	CTTGGGCGGCTTTAATGGTA	TGCTACTGCACTGTTCATTCT	50.0	42.9	58.2	57.6	1147-1163 bp	1.0	68.7
petN-psbM_p2	TTGGGCGGCTTTAATGGTAG	TGCTACTGCACTGTTCATTCT	50.0	42.9	58.2	57.6	1146-1162 bp	1.0	68.7
petN-psbM_p3	GGCGGCTTTAATGGTAGTCT	TGCTACTGCACTGTTCATTCT	50.0	42.9	57.7	57.6	1143-1159 bp	1.0	68.1
petN-psbM_p4	ATGGGGAAGAAGTGGACTCT	TGCTACTGCACTGTTCATTCT	50.0	42.9	57.6	57.6	1098-1114 bp	1.0	68.0
petN-psbM_p5	TAGTAAGTCTTGCTTGGGCG	TGCTACTGCACTGTTCATTCT	50.0	42.9	57.6	57.6	1159-1175 bp	1.0	67.6