Hotspot

trnS-GCU-trnG-UCC

Genome · 9690-10581 · 892 bp

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Pi 0.0106

MarkerSeek score 51.60

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: Genome
length_bp: 892
variable_sites: 33
pi: 0.0106
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 51.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	GCTTTAGTCCACTCAGCCAT	TGAATCAAACCGAAAGATCCCT	50.0	40.9	57.6	57.7	968-1085 bp	1.0	65.0
trnS-GCU-trnG-UCC_p2	AGTCCACTCAGCCATCTCTC	TGAATCAAACCGAAAGATCCCT	55.0	40.9	58.5	57.7	963-1080 bp	1.0	64.1
trnS-GCU-trnG-UCC_p3	TAGTCCACTCAGCCATCTCT	TGAATCAAACCGAAAGATCCCT	50.0	40.9	57.2	57.7	964-1081 bp	1.0	61.6
trnS-GCU-trnG-UCC_p4	GCTTTAGTCCACTCAGCCAT	TGAATCAAACCGAAAGATCCC	50.0	42.9	57.6	56.2	968-1085 bp	1.0	61.2
trnS-GCU-trnG-UCC_p5	AGTCCACTCAGCCATCTCTC	TGAATCAAACCGAAAGATCCC	55.0	42.9	58.5	56.2	963-1080 bp	1.0	60.4