Hotspot

trnQ-UUG-psbK

Genome · 8425-8764 · 340 bp

Back to Illicium

Pi 0.017

MarkerSeek score 61.40

Primer available yes

Label trnQ-UUG-psbK

Feature Metadata

label_name: trnQ-UUG-psbK
feature_type: igs
region: Genome
length_bp: 340
variable_sites: 22
pi: 0.017
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnQ-UUG-psbK_p1	GAATTAGCCTGGGACGGAAG	GGGCAATTTGGCGAAGAAAA	55.0	45.0	58.0	57.8	491-511 bp	1.0	84.7
trnQ-UUG-psbK_p2	GAAGGATTCGAACCTCCGAG	GGGCAATTTGGCGAAGAAAA	55.0	45.0	57.8	57.8	475-495 bp	1.0	83.6
trnQ-UUG-psbK_p3	CAAAACCTGTTGCCTTACCG	GGGCAATTTGGCGAAGAAAA	50.0	45.0	57.6	57.8	446-466 bp	1.0	82.4
trnQ-UUG-psbK_p4	AGGATTCGAACCTCCGAGTA	GGGCAATTTGGCGAAGAAAA	50.0	45.0	57.6	57.8	473-493 bp	1.0	81.9
trnQ-UUG-psbK_p5	AGAATTAGCCTGGGACGGAA	GGGCAATTTGGCGAAGAAAA	50.0	45.0	58.4	57.8	492-512 bp	1.0	81.7