Hotspot

rps16-trnQ-UUG

Genome · 6779-8352 · 1574 bp

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Pi 0.00754

MarkerSeek score 61.70

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: Genome
length_bp: 1574
variable_sites: 40
pi: 0.00754
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	ATCATGTCGTTCAAGTCGCA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	57.9	58.1	1653-1765 bp	1.0	84.6
rps16-trnQ-UUG_p2	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	1641-1753 bp	1.0	84.2
rps16-trnQ-UUG_p3	ATCATGTCGTTCAAGTCGCA	CTCGGAGGTTCGAATCCTTC	45.0	55.0	57.9	57.8	1657-1769 bp	1.0	83.5
rps16-trnQ-UUG_p4	AAGTCGCACGTTGCTTTCTA	CTCGGAGGTTCGAATCCTTC	45.0	55.0	58.1	57.8	1645-1757 bp	1.0	83.1
rps16-trnQ-UUG_p5	ATCATGTCGTTCAAGTCGCA	CGGTAAGGCAACAGGTTTTG	45.0	50.0	57.9	57.6	1686-1798 bp	1.0	81.9