Hotspot

rbcL

LSC · 57626-59053 · 1428 bp

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Pi 0.00836
MarkerSeek score 53.60
Primer available yes
Label rbcL

Feature Metadata

label_name
rbcL
feature_type
gene
region
LSC
length_bp
1428
variable_sites
62
pi
0.00836
unique_haplotype_count
43
primer_available
yes
alignment_reliability
1.00
markerseek_score
53.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rbcL_p1 TCATGAGTTGTAGGGAGGGA TGGGCCGAGTTTAATTGCAA 50.0 45.0 57.1 58.4 1503 bp 1.0 72.8
rbcL_p2 TCATGAGTTGTAGGGAGGGA TTGGGCCGAGTTTAATTGCA 50.0 45.0 57.1 58.4 1504 bp 1.0 72.7
rbcL_p3 ACGCCTAATTCATGTCGAGT TGGGCCGAGTTTAATTGCAA 45.0 45.0 57.0 58.4 1551 bp 1.0 72.6
rbcL_p4 ACGCCTAATTCATGTCGAGT TTGGGCCGAGTTTAATTGCA 45.0 45.0 57.0 58.4 1552 bp 1.0 72.6
rbcL_p5 TTCATGAGTTGTAGGGAGGGA TGGGCCGAGTTTAATTGCAA 47.6 45.0 57.8 58.4 1504 bp 1.0 70.7