Hotspot

psaC

SSC · 120656-120901 · 246 bp

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Pi 0.00196
MarkerSeek score 23.10
Primer available yes
Label psaC

Feature Metadata

label_name
psaC
feature_type
gene
region
SSC
length_bp
246
variable_sites
5
pi
0.00196
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
23.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaC_p1 GGAACCTGCAGATATTGGCA GCCGCGTAAGGTATGATCAT 50.0 50.0 57.9 57.9 632-641 bp 0.980198 84.4
psaC_p2 TGCATAGGTCGTCAGAAGGA GCCGCGTAAGGTATGATCAT 50.0 50.0 58.2 57.9 725-734 bp 0.940594 81.8
psaC_p3 TCGAGCACGGATTTTATCGG GCCGCGTAAGGTATGATCAT 50.0 50.0 58.2 57.9 509-517 bp 0.980198 81.6
psaC_p4 GAATCTCGAGTAACTGGCCG GCCGCGTAAGGTATGATCAT 55.0 50.0 58.2 57.9 907-916 bp 0.920792 80.0
psaC_p5 CGTTAGTAAAATGGGCCCGA GCCGCGTAAGGTATGATCAT 50.0 50.0 58.0 57.9 855-857 bp 0.722772 72.3