Hotspot

ndhF-rpl32

SSC · 115653-116605 · 953 bp

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Pi 0.00818
MarkerSeek score 56.00
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
953
variable_sites
79
pi
0.00818
unique_haplotype_count
58
primer_available
yes
alignment_reliability
1.00
markerseek_score
56.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 GCATATTGATATGTCTGTTCCA TTAACGCTGCCCAATATCCC 36.4 50.0 54.1 58.0 1041-1073 bp 1.0 50.1
ndhF-rpl32_p2 GCATATTGATATGTCTGTTCCA AAAAGCTTTTAACGCTGCCC 36.4 45.0 54.1 57.9 1049-1081 bp 1.0 50.1
ndhF-rpl32_p3 GCATATTGATATGTCTGTTCCAT TTAACGCTGCCCAATATCCC 34.8 50.0 54.6 58.0 1041-1073 bp 1.0 50.1
ndhF-rpl32_p4 GCATATTGATATGTCTGTTCCA GCCCAATATCCCTTCCGTTT 36.4 50.0 54.1 57.9 1033-1065 bp 0.980198 49.2
ndhF-rpl32_p5 GCATATTGATATGTCTGTTCCAT GCCCAATATCCCTTCCGTTT 34.8 50.0 54.6 57.9 1033-1065 bp 0.980198 49.2