Hotspot

ndhC-trnV-UAC

LSC · 52673-53848 · 1176 bp

Back to Ilex

Pi 0.00783
MarkerSeek score 68.60
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1176
variable_sites
105
pi
0.00783
unique_haplotype_count
58
primer_available
yes
alignment_reliability
0.99
markerseek_score
68.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 GGGGCTAAAACTCCGGAAAT AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.9 57.8 1265-1308 bp 0.990099 87.5
ndhC-trnV-UAC_p2 GTCCTTTGCTAATTGGGGCT AGAAGGTCTACGGTTCGAGT 50.0 50.0 58.1 57.8 1279-1322 bp 0.990099 87.4
ndhC-trnV-UAC_p3 AATTGGGGCTAAAACTCCGG AGAAGGTCTACGGTTCGAGT 50.0 50.0 58.2 57.8 1269-1312 bp 0.990099 87.4
ndhC-trnV-UAC_p4 TGGTCCTTTGCTAATTGGGG AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.8 57.8 1281-1324 bp 0.990099 86.7
ndhC-trnV-UAC_p5 GGGGCTAAAACTCCGGAAAT GAAGGTCTACGGTTCGAGTC 50.0 55.0 57.9 57.5 1264-1307 bp 0.990099 84.8