Hotspot

trnS-UGA-lhbA

LSC · 37560-37913 · 354 bp

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Pi 0.00943
MarkerSeek score 62.10
Primer available yes
Label trnS-UGA-lhbA

Feature Metadata

label_name
trnS-UGA-lhbA
feature_type
igs
region
LSC
length_bp
354
variable_sites
32
pi
0.00943
unique_haplotype_count
51
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-UGA-lhbA_p1 CAACCACTCAGCCATCTCTC CCAACCATCAGGAGAAGCAA 55.0 50.0 58.0 57.8 468-488 bp 1.0 86.4
trnS-UGA-lhbA_p2 GAGCTATCAACCACTCAGCC CCAACCATCAGGAGAAGCAA 55.0 50.0 58.1 57.8 475-495 bp 1.0 86.0
trnS-UGA-lhbA_p3 GGAGCTATCAACCACTCAGC CCAACCATCAGGAGAAGCAA 55.0 50.0 58.1 57.8 476-496 bp 1.0 86.0
trnS-UGA-lhbA_p4 CAACCACTCAGCCATCTCTC GACCAACCATCAGGAGAAGC 55.0 55.0 58.0 58.3 470-490 bp 1.0 86.0
trnS-UGA-lhbA_p5 CAAGACCGGAGCTATCAACC CCAACCATCAGGAGAAGCAA 55.0 50.0 58.1 57.8 483-503 bp 1.0 85.9