Hotspot

trnC-GCA-petN

LSC · 29234-30115 · 882 bp

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Pi 0.0049
MarkerSeek score 60.20
Primer available yes
Label trnC-GCA-petN

Feature Metadata

label_name
trnC-GCA-petN
feature_type
igs
region
LSC
length_bp
882
variable_sites
60
pi
0.0049
unique_haplotype_count
47
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnC-GCA-petN_p1 TTTCCCCAGTTCAAATCCGG AGCCCAAGCGAGACTTACTA 50.0 50.0 58.1 58.2 761-949 bp 1.0 87.2
trnC-GCA-petN_p2 TTGTATCCTTTTGGCGGCAT AGCCCAAGCGAGACTTACTA 45.0 50.0 58.2 58.2 815-1003 bp 1.0 86.4
trnC-GCA-petN_p3 CTTTGTATCCTTTTGGCGGC AGCCCAAGCGAGACTTACTA 50.0 50.0 57.7 58.2 817-1005 bp 1.0 85.4
trnC-GCA-petN_p4 TTTCCCCAGTTCAAATCCGG AGAGTCCACTTCTTCCCCAT 50.0 50.0 58.1 57.6 822-1010 bp 1.0 85.3
trnC-GCA-petN_p5 TTGTATCCTTTTGGCGGCAT AGAGTCCACTTCTTCCCCAT 45.0 50.0 58.2 57.6 876-1064 bp 1.0 84.5