Hotspot

atpH-atpI

LSC · 14488-15625 · 1138 bp

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Pi 0.00687
MarkerSeek score 61.60
Primer available yes
Label atpH-atpI

Feature Metadata

label_name
atpH-atpI
feature_type
igs
region
LSC
length_bp
1138
variable_sites
83
pi
0.00687
unique_haplotype_count
51
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpH-atpI_p1 ATAACGGAAGCGGCAGAAAT TTTTTGCAACTTTAGCCGCG 45.0 45.0 58.0 58.5 1209-1234 bp 1.0 83.8
atpH-atpI_p2 GCCAACTCCAGGTCCAATAG TTTTTGCAACTTTAGCCGCG 55.0 45.0 58.0 58.5 1258-1283 bp 1.0 83.4
atpH-atpI_p3 AGCCAATCCAGCAGCAATAA TTTTTGCAACTTTAGCCGCG 45.0 45.0 57.9 58.5 1225-1250 bp 1.0 82.8
atpH-atpI_p4 AATAACGGAAGCGGCAGAAA TTTTTGCAACTTTAGCCGCG 45.0 45.0 58.2 58.5 1210-1235 bp 1.0 82.6
atpH-atpI_p5 GCCAATCCAGCAGCAATAAC TTTTTGCAACTTTAGCCGCG 50.0 45.0 57.8 58.5 1224-1249 bp 1.0 82.3