Hotspot

rps16-trnQ-UUG

LSC · 5438-7795 · 2358 bp

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Pi 0.00852
MarkerSeek score 70.30
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
2358
variable_sites
207
pi
0.00852
unique_haplotype_count
82
primer_available
yes
alignment_reliability
1.00
markerseek_score
70.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 TTCCTTCTCTTCGGGATCGA GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.9 58.1 2294-2424 bp 1.0 89.2
rps16-trnQ-UUG_p2 CGGGATCGAACATCAATTGC GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.6 58.1 2283-2413 bp 1.0 87.2
rps16-trnQ-UUG_p3 CTTCCTTCTCTTCGGGATCG GAGGTTCGAATCCTTCCGTC 55.0 55.0 57.5 58.1 2295-2425 bp 1.0 86.3
rps16-trnQ-UUG_p4 TTCCTTCTCTTCGGGATCGA TTCGGAGGTTCGAATCCTTC 50.0 50.0 57.9 57.3 2298-2428 bp 1.0 84.4
rps16-trnQ-UUG_p5 TTCCTTCTCTTCGGGATCGA TAAGGCAACGGGTTTTGGTC 50.0 50.0 57.9 58.7 2324-2454 bp 0.990099 83.9