Hotspot

orf244

Genome · 66324-67058 · 735 bp

Pi 0.205

MarkerSeek score 84.70

Primer available yes

Label orf244

Feature Metadata

label_name: orf244
feature_type: gene
region: Genome
length_bp: 735
variable_sites: 336
pi: 0.205
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 84.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
orf244_p1	TATGCATCTGGCTGGATTCG	AAAATGCAGACGTATTGCGC	50.0	45.0	57.8	58.1	955-1185 bp	0.857143	83.5
orf244_p2	TATGCATCTGGCTGGATTCG	CTACTAAGCATCTGTGGCCG	50.0	55.0	57.8	58.1	1499-1627 bp	0.285714	56.9
orf244_p3	ACCAGCGACGTCCTTTTAAG	AAAATGCAGACGTATTGCGC	50.0	45.0	58.2	58.1	934 bp	0.142857	43.2
orf244_p4	GAACCAGCGACGTCCTTTTA	AAAATGCAGACGTATTGCGC	50.0	45.0	58.2	58.1	936 bp	0.142857	43.2
orf244_p5	AACCAGCGACGTCCTTTTAA	AAAATGCAGACGTATTGCGC	45.0	45.0	57.8	58.1	935 bp	0.142857	42.9