Hotspot

dnaB

Genome · 151249-153054 · 1806 bp

Back to Hypnea

Pi 0.16
MarkerSeek score 79.50
Primer available yes
Label dnaB

Feature Metadata

label_name
dnaB
feature_type
gene
region
Genome
length_bp
1806
variable_sites
658
pi
0.16
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
79.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
dnaB_p1 TCAGTCCACTGCTCTACCAA CAGACATTGCAACTCCTGGT 50.0 50.0 58.0 58.1 2410-2510 bp 0.571429 73.6
dnaB_p2 TCCACTGCTCTACCAACTGA CAGACATTGCAACTCCTGGT 50.0 50.0 58.0 58.1 2406-2506 bp 0.571429 73.6
dnaB_p3 TTCAGTCCACTGCTCTACCA CAGACATTGCAACTCCTGGT 50.0 50.0 58.0 58.1 2411-2511 bp 0.571429 73.6
dnaB_p4 AGTCCACTGCTCTACCAACT CAGACATTGCAACTCCTGGT 50.0 50.0 58.0 58.1 2408-2508 bp 0.571429 73.5
dnaB_p5 TTTGAACTGGTGACACGAGG CAGACATTGCAACTCCTGGT 50.0 50.0 58.1 58.1 2434-2534 bp 0.571429 73.2