Hotspot

groEL-ycf65

Genome · 166958-167499 · 542 bp

Back to Hypnea

Pi 0.219
MarkerSeek score 83.20
Primer available yes
Label groEL-ycf65

Feature Metadata

label_name
groEL-ycf65
feature_type
igs
region
Genome
length_bp
542
variable_sites
257
pi
0.219
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.99
markerseek_score
83.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
groEL-ycf65_p1 AAAGGTTACCCGTTCTGCTG GGTGCACAAATGACACCAAA 50.0 45.0 58.1 57.4 985-1005 bp 0.428571 61.2
groEL-ycf65_p2 AGCAAAGGTTACCCGTTCTG GGTGCACAAATGACACCAAA 50.0 45.0 58.1 57.4 988-1008 bp 0.428571 61.2
groEL-ycf65_p3 CAAAGGTTACCCGTTCTGCT GGTGCACAAATGACACCAAA 50.0 45.0 58.1 57.4 986-1006 bp 0.428571 61.2
groEL-ycf65_p4 CAGCAAAGGTTACCCGTTCT GGTGCACAAATGACACCAAA 50.0 45.0 58.1 57.4 989-1009 bp 0.428571 61.2
groEL-ycf65_p5 GTTACCCGTTCTGCTGTTCA GGTGCACAAATGACACCAAA 50.0 45.0 58.1 57.4 1001 bp 0.142857 40.0