Hotspot

orf244-trnMe(cat)

Genome · 67059-67620 · 562 bp

Back to Hypnea

Pi 0.127
MarkerSeek score 84.10
Primer available yes
Label orf244-trnMe(cat)

Feature Metadata

label_name
orf244-trnMe(cat)
feature_type
igs
region
Genome
length_bp
562
variable_sites
163
pi
0.127
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
84.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
orf244-trnMe(cat)_p1 AGAGGGCAACGACTGAATTC CTACTAAGCATCTGTGGCCG 50.0 55.0 57.9 58.1 1159 bp 0.142857 43.8
orf244-trnMe(cat)_p2 GAGGGCAACGACTGAATTCT CTACTAAGCATCTGTGGCCG 50.0 55.0 57.9 58.1 1158 bp 0.142857 43.8
orf244-trnMe(cat)_p3 AGAGGGCAACGACTGAATTC CAGGAAGAACGAGCATTTGC 50.0 50.0 57.9 57.7 1761 bp 0.142857 42.9
orf244-trnMe(cat)_p4 GAGGGCAACGACTGAATTCT CAGGAAGAACGAGCATTTGC 50.0 50.0 57.9 57.7 1760 bp 0.142857 42.9
orf244-trnMe(cat)_p5 GAGGGCAACGACTGAATTCT TACTAAGCATCTGTGGCCGA 50.0 50.0 57.9 58.5 1157 bp 0.142857 40.8