Hotspot

apcF-trnR(ccg)

Genome · 55219-55694 · 476 bp

Back to Hypnea

Pi 0.176
MarkerSeek score 82.00
Primer available yes
Label apcF-trnR(ccg)

Feature Metadata

label_name
apcF-trnR(ccg)
feature_type
igs
region
Genome
length_bp
476
variable_sites
183
pi
0.176
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
82.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
apcF-trnR(ccg)_p1 CTTGCTGCCGCTTCTTTAAC ATCCACTGAGTTACATGCCC 50.0 50.0 58.0 57.3 669 bp 0.142857 39.7
apcF-trnR(ccg)_p2 TGATTTTAGCGCAGGCGTAT ATCCACTGAGTTACATGCCC 45.0 50.0 58.1 57.3 949 bp 0.142857 39.6
apcF-trnR(ccg)_p3 TCTTGCTGCCGCTTCTTTAA ATCCACTGAGTTACATGCCC 45.0 50.0 58.1 57.3 670 bp 0.142857 39.1
apcF-trnR(ccg)_p4 ATCTTGCTGCCGCTTCTTTA ATCCACTGAGTTACATGCCC 45.0 50.0 57.9 57.3 671 bp 0.142857 39.1
apcF-trnR(ccg)_p5 TAATCTTGCTGCCGCTTCTT ATCCACTGAGTTACATGCCC 45.0 50.0 57.9 57.3 673 bp 0.142857 39.1