Hotspot

ycf21-pgmA

Genome · 39543-40023 · 481 bp

Back to Hypnea

Pi 0.173
MarkerSeek score 80.40
Primer available yes
Label ycf21-pgmA

Feature Metadata

label_name
ycf21-pgmA
feature_type
igs
region
Genome
length_bp
481
variable_sites
185
pi
0.173
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.99
markerseek_score
80.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf21-pgmA_p1 GCGCACGTCTAATAGCTTCT TACATCAGACCACGGCAATG 50.0 50.0 58.2 58.0 1028-1037 bp 0.285714 58.7
ycf21-pgmA_p2 CACTGGTCATGGATTGCTGA CATTACATCAGACCACGGCA 50.0 50.0 57.9 58.0 1295 bp 0.142857 44.6
ycf21-pgmA_p3 CACTGGTCATGGATTGCTGA TACATCAGACCACGGCAATG 50.0 50.0 57.9 58.0 1292 bp 0.142857 44.6
ycf21-pgmA_p4 TCACTGGTCATGGATTGCTG CATTACATCAGACCACGGCA 50.0 50.0 57.9 58.0 1296 bp 0.142857 44.6
ycf21-pgmA_p5 TCACTGGTCATGGATTGCTG TACATCAGACCACGGCAATG 50.0 50.0 57.9 58.0 1293 bp 0.142857 44.6