Hotspot

rpl16

LSC · 78595-80041 · 1447 bp

Back to Hyparrhenia

Pi 0.0109

MarkerSeek score 55.30

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1447
variable_sites: 92
pi: 0.0109
unique_haplotype_count: 29
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 55.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	GATTACCAGCGGCTCCTATG	GGGTCGTCTAGCAGGAAAAG	55.0	55.0	57.9	58.0	1950-2018 bp	1.0	87.4
rpl16_p2	GATTACCAGCGGCTCCTATG	AACCATGTCTTCCCATTCCG	55.0	50.0	57.9	57.9	1785-1853 bp	1.0	87.1
rpl16_p3	TTATACCGTCGTCGCCTTTG	GGGTCGTCTAGCAGGAAAAG	50.0	55.0	58.1	58.0	2088-2156 bp	1.0	87.0
rpl16_p4	TTTCTAGAGGCATTTGGGGC	GGGTCGTCTAGCAGGAAAAG	50.0	55.0	57.9	58.0	2022-2090 bp	1.0	86.7
rpl16_p5	TCAGCAATAGTGTCCTTGCC	GGGTCGTCTAGCAGGAAAAG	50.0	55.0	57.9	58.0	2393-2461 bp	1.0	86.2