Hotspot

ccsA

SSC · 108084-109049 · 966 bp

Back to Hyparrhenia

Pi 0.00506

MarkerSeek score 42.50

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 966
variable_sites: 31
pi: 0.00506
unique_haplotype_count: 24
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 42.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	GCGGCATTCTCGAAAAAGAA	CCTGAAGGTTTTATGTTATGTAA	45.0	30.4	57.4	52.5	1072 bp	0.918919	39.1
ccsA_p2	CTCAAGCATCTCGGTTCGAA	CCTGAAGGTTTTATGTTATGTAA	50.0	30.4	58.0	52.5	1100 bp	0.918919	39.0
ccsA_p3	AAGCATCTCGGTTCGAATCC	CCTGAAGGTTTTATGTTATGTAA	50.0	30.4	58.1	52.5	1097 bp	0.918919	39.0
ccsA_p4	TCAAGCATCTCGGTTCGAAT	CCTGAAGGTTTTATGTTATGTAA	45.0	30.4	57.3	52.5	1099 bp	0.918919	39.0
ccsA_p5	CAAGCATCTCGGTTCGAATC	CCTGAAGGTTTTATGTTATGTAA	50.0	30.4	57.0	52.5	1098 bp	0.918919	39.0