Hotspot

ndhF-rpl32

SSC · 106347-107208 · 862 bp

Back to Hyparrhenia

Pi 0.00946

MarkerSeek score 67.70

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 862
variable_sites: 59
pi: 0.00946
unique_haplotype_count: 29
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	CTGGAAGTGGGAGAAGAGGA	TCCAAAGATTTTTACGAATACGCT	55.0	33.3	58.1	57.3	940-1002 bp	1.0	50.6
ndhF-rpl32_p2	CTGGAAGTGGGAGAAGAGGA	TGACATCGAAGTACGTTTTT	55.0	35.0	58.1	53.2	912-974 bp	1.0	50.5
ndhF-rpl32_p3	CTGGAAGTGGGAGAAGAGGA	TTGACATCGAAGTACGTTTT	55.0	35.0	58.1	53.2	913-975 bp	1.0	50.5
ndhF-rpl32_p4	CTGGAAGTGGGAGAAGAGGA	TTTGACATCGAAGTACGTTT	55.0	35.0	58.1	53.2	914-976 bp	1.0	50.5
ndhF-rpl32_p5	CTGGAAGTGGGAGAAGAGGA	TTTTGACATCGAAGTACGTT	55.0	35.0	58.1	53.2	915-977 bp	1.0	50.5