Hotspot

psaJ-rpl33

LSC · 65735-66100 · 366 bp

Back to Hyparrhenia

Pi 0.0125

MarkerSeek score 61.00

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 366
variable_sites: 24
pi: 0.0125
unique_haplotype_count: 20
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	TTTGGGGCTTTAGCAGGTTT	CCCCGTCGATTCCTTATTGG	45.0	55.0	57.9	58.1	513-575 bp	1.0	85.4
psaJ-rpl33_p2	ATGGTTTGGGGCTTTAGCAG	CCCCGTCGATTCCTTATTGG	50.0	55.0	58.4	58.1	517-579 bp	1.0	82.6
psaJ-rpl33_p3	TTTGGGGCTTTAGCAGGTTT	GAAATCCCCGTCGATTCCTT	45.0	50.0	57.9	57.7	518-580 bp	0.972973	82.5
psaJ-rpl33_p4	TTTGGGGCTTTAGCAGGTTT	CCCGTCGATTCCTTATTGGT	45.0	50.0	57.9	57.4	512-574 bp	1.0	81.5
psaJ-rpl33_p5	TTTGGGGCTTTAGCAGGTTT	AGAAATCCCCGTCGATTCCT	45.0	50.0	57.9	58.5	519-581 bp	0.972973	81.0