Hotspot

trnF-GAA-ndhJ

LSC · 48851-49468 · 618 bp

Back to Hyparrhenia

Pi 0.01

MarkerSeek score 63.00

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 618
variable_sites: 44
pi: 0.01
unique_haplotype_count: 25
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	TGAAAGTTGGATAGGCTGGC	50.0	50.0	58.1	57.9	706-760 bp	1.0	87.5
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	ATAGGCTGGCCCTTACGTAA	50.0	50.0	58.1	58.2	696-750 bp	1.0	87.0
trnF-GAA-ndhJ_p3	ATGCACAAGAAAGGGTCAGG	TGAAAGTTGGATAGGCTGGC	50.0	50.0	58.1	57.9	759-813 bp	1.0	86.9
trnF-GAA-ndhJ_p4	ATGCACAAGAAAGGGTCAGG	ATAGGCTGGCCCTTACGTAA	50.0	50.0	58.1	58.2	749-803 bp	1.0	86.4
trnF-GAA-ndhJ_p5	GCACAAGAAAGGGTCAGGAT	TGAAAGTTGGATAGGCTGGC	50.0	50.0	57.8	57.9	757-811 bp	1.0	85.9