Hotspot

trnC-GCA-rpoB

LSC · 19042-20275 · 1234 bp

Back to Hyparrhenia

Pi 0.00845

MarkerSeek score 63.10

Primer available yes

Label trnC-GCA-rpoB

Feature Metadata

label_name: trnC-GCA-rpoB
feature_type: igs
region: LSC
length_bp: 1234
variable_sites: 88
pi: 0.00845
unique_haplotype_count: 32
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-rpoB_p1	AAAGGATTTGCAGTCCCCTG	CTTCTCAAGTTCTTCCGCCA	50.0	50.0	58.1	57.8	1351-1507 bp	1.0	87.2
trnC-GCA-rpoB_p2	AAAGGATTTGCAGTCCCCTG	TCTCAAGTTCTTCCGCCAAG	50.0	50.0	58.1	57.8	1349-1505 bp	1.0	87.2
trnC-GCA-rpoB_p3	AAAGGATTTGCAGTCCCCTG	ACTTCTCAAGTTCTTCCGCC	50.0	50.0	58.1	57.8	1352-1508 bp	1.0	87.2
trnC-GCA-rpoB_p4	GATTTTTGATCAGGCGGCAC	CTTCTCAAGTTCTTCCGCCA	50.0	50.0	58.1	57.8	1390-1546 bp	1.0	87.1
trnC-GCA-rpoB_p5	GATTTTTGATCAGGCGGCAC	TCTCAAGTTCTTCCGCCAAG	50.0	50.0	58.1	57.8	1388-1544 bp	1.0	87.1