Hotspot

trnD-GUC-psbM

LSC · 17015-17699 · 685 bp

Back to Hyparrhenia

Pi 0.00512

MarkerSeek score 59.10

Primer available yes

Label trnD-GUC-psbM

Feature Metadata

label_name: trnD-GUC-psbM
feature_type: igs
region: LSC
length_bp: 685
variable_sites: 46
pi: 0.00512
unique_haplotype_count: 23
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-psbM_p1	AATAGGCATGCCATACACCC	GGCAGTAGGAACTAGAATGAACA	50.0	43.5	57.7	57.9	829-1222 bp	1.0	60.5
trnD-GUC-psbM_p2	TTCAATTGGTCAGAGCACCG	GGCAGTAGGAACTAGAATGAACA	50.0	43.5	58.5	57.9	795-1188 bp	1.0	59.3
trnD-GUC-psbM_p3	AATAGGCATGCCATACACCC	AGGCAGTAGGAACTAGAATGAAC	50.0	43.5	57.7	57.7	830-1223 bp	1.0	58.4
trnD-GUC-psbM_p4	AATAGGCATGCCATACACCC	TGCGAGAATATTGACTTCCA	50.0	40.0	57.7	54.5	790-1183 bp	1.0	57.3
trnD-GUC-psbM_p5	AATAGGCATGCCATACACCC	AGGCAGTAGGAACTAGAATGA	50.0	42.9	57.7	55.5	830-1223 bp	1.0	57.1