Hotspot

ycf1

SSC · 109197-114254 · 5058 bp

Back to Hyoseris

Pi 0.000991

MarkerSeek score 53.30

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5058
variable_sites: 5
pi: 0.000991
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GGCCCTCCTATCTCTTCCTT	ACGCATTGAATTTCGGTTAGT	55.0	38.1	57.9	56.5	263 bp	1.0	59.7
ycf1_p2	GGCCCTCCTATCTCTTCCTT	TGCTGAGATTACGCATTGAA	55.0	40.0	57.9	55.5	273 bp	1.0	59.2
ycf1_p3	GGCCCTCCTATCTCTTCCTT	TTGAATGCTGAGATTACGCA	55.0	40.0	57.9	55.5	278 bp	1.0	59.2
ycf1_p4	GGCCCTCCTATCTCTTCCTT	CATTGAATGCTGAGATTACGCA	55.0	40.9	57.9	57.5	280 bp	1.0	59.1
ycf1_p5	GGCCCTCCTATCTCTTCCTT	ACATTGAATGCTGAGATTACGC	55.0	40.9	57.9	57.5	281 bp	1.0	59.1