Hotspot

accD

LSC · 57533-59011 · 1479 bp

Back to Hyoseris

Pi 0.00135

MarkerSeek score 51.70

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 1479
variable_sites: 2
pi: 0.00135
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	TTTTCAATACTCACACTCCTT	ACAAAGAAAACCCCATTCGTC	33.3	42.9	52.7	57.0	1667 bp	1.0	40.1
accD_p2	TTTTTCAATACTCACACTCCT	ACAAAGAAAACCCCATTCGTC	33.3	42.9	52.7	57.0	1668 bp	1.0	40.1
accD_p3	TTTTCAATACTCACACTCCTT	CAACAAAGAAAACCCCATTCGT	33.3	40.9	52.7	57.9	1669 bp	1.0	40.1
accD_p4	TTTTTCAATACTCACACTCCT	CAACAAAGAAAACCCCATTCGT	33.3	40.9	52.7	57.9	1670 bp	1.0	40.1
accD_p5	TTTTTCAATACTCACACTCCTT	ACAAAGAAAACCCCATTCGTC	31.8	42.9	53.6	57.0	1668 bp	1.0	40.1