Hotspot

ndhF-trnN-GUU

IRa · 127944-128745 · 802 bp

Back to Hyoseris

Pi 0.00125

MarkerSeek score 59.50

Primer available yes

Label ndhF-trnN-GUU

Feature Metadata

label_name: ndhF-trnN-GUU
feature_type: igs
region: IRa
length_bp: 802
variable_sites: 1
pi: 0.00125
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-trnN-GUU_p1	GGGTGGGCGTATTTCTTCTT	AGGATTCGAACCTACGACCA	50.0	50.0	57.9	58.2	950 bp	1.0	77.8
ndhF-trnN-GUU_p2	GGGTGGGCGTATTTCTTCTT	ACCACTGAGCTACTGAGGAA	50.0	50.0	57.9	57.7	907 bp	1.0	76.6
ndhF-trnN-GUU_p3	GGGTGGGCGTATTTCTTCTT	CGAGGTGTTGACGGTGATTT	50.0	50.0	57.9	58.5	986 bp	1.0	75.2
ndhF-trnN-GUU_p4	GGGTGGGCGTATTTCTTCTT	GGATTCGAACCTACGACCAA	50.0	50.0	57.9	57.3	949 bp	1.0	73.8
ndhF-trnN-GUU_p5	GGGTGGGCGTATTTCTTCTT	GAGTATCCCGGACCTACACC	50.0	60.0	57.9	58.7	1005 bp	1.0	73.7