Hotspot

rpl32-ndhF

SSC · 124619-125711 · 1093 bp

Back to Hyoseris

Pi 0.00275

MarkerSeek score 60.40

Primer available yes

Label rpl32-ndhF

Feature Metadata

label_name: rpl32-ndhF
feature_type: igs
region: SSC
length_bp: 1093
variable_sites: 3
pi: 0.00275
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-ndhF_p1	CGGCCCAATATCCTTTCCTT	GTTGCCGTCGGAAAAAGAAG	50.0	50.0	57.6	58.0	1272-1273 bp	1.0	77.1
rpl32-ndhF_p2	CGGCCCAATATCCTTTCCTT	TGCCGTCGGAAAAAGAAGAA	50.0	45.0	57.6	58.0	1270-1271 bp	1.0	77.0
rpl32-ndhF_p3	CGGCCCAATATCCTTTCCTT	TTGCCGTCGGAAAAAGAAGA	50.0	45.0	57.6	58.0	1271-1272 bp	1.0	77.0
rpl32-ndhF_p4	CGGCCCAATATCCTTTCCTT	CTTTTTGTTGCCGTCGGAAA	50.0	45.0	57.6	57.8	1278-1279 bp	1.0	75.8
rpl32-ndhF_p5	CGGCCCAATATCCTTTCCTT	TTTGTTGCCGTCGGAAAAAG	50.0	45.0	57.6	57.8	1275-1276 bp	1.0	75.8