Hotspot

trnL-UAA-trnF-GAA

LSC · 47619-48358 · 740 bp

Back to Hyoseris

Pi 0.00372

MarkerSeek score 57.50

Primer available yes

Label trnL-UAA-trnF-GAA

Feature Metadata

label_name: trnL-UAA-trnF-GAA
feature_type: igs
region: LSC
length_bp: 740
variable_sites: 2
pi: 0.00372
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.73
markerseek_score: 57.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAA-trnF-GAA_p1	AATCGTGAGGGTTCAAGTCC	TTTGAACTGGTGACACGAGG	50.0	50.0	57.5	58.1	628-830 bp	1.0	80.0
trnL-UAA-trnF-GAA_p2	AATCGTGAGGGTTCAAGTCC	GAACTGGTGACACGAGGATT	50.0	50.0	57.5	57.5	625-827 bp	1.0	76.8
trnL-UAA-trnF-GAA_p3	TTCAAGTCCCTCTATCCCCA	TTTGAACTGGTGACACGAGG	50.0	50.0	57.0	58.1	617-819 bp	1.0	76.1
trnL-UAA-trnF-GAA_p4	AATCGTGAGGGTTCAAGTCC	TGAACTGGTGACACGAGGAT	50.0	50.0	57.5	58.7	626-828 bp	1.0	75.1
trnL-UAA-trnF-GAA_p5	AGAGTCCCGTTCTACATGTCA	TTTGAACTGGTGACACGAGG	47.6	50.0	58.2	58.1	700-902 bp	1.0	74.2