Hotspot

trnT-GGU-psbD

LSC · 31323-32557 · 1235 bp

Back to Hyoseris

Pi 0.00081

MarkerSeek score 59.20

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1235
variable_sites: 1
pi: 0.00081
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	CGTAAGCCCTCGGTTCAAAT	GGACCAGCCTACAAAAACGA	50.0	50.0	58.3	58.1	1370 bp	1.0	77.0
trnT-GGU-psbD_p2	TCCATGAACCAAGCCCTTTT	GGACCAGCCTACAAAAACGA	45.0	50.0	57.6	58.1	1426 bp	1.0	76.0
trnT-GGU-psbD_p3	TTCCATGAACCAAGCCCTTT	GGACCAGCCTACAAAAACGA	45.0	50.0	57.6	58.1	1427 bp	1.0	76.0
trnT-GGU-psbD_p4	CGTAAGCCCTCGGTTCAAAT	ACAAAAACGAAACGGTCCCT	50.0	45.0	58.3	58.2	1360 bp	1.0	76.0
trnT-GGU-psbD_p5	TAAGCCCTCGGTTCAAATCC	GGACCAGCCTACAAAAACGA	50.0	50.0	57.6	58.1	1368 bp	1.0	75.8