Hotspot

trnR-UCU-trnT-GGU

LSC · 30058-31245 · 1188 bp

Back to Hyoseris

Pi 0.00085

MarkerSeek score 56.20

Primer available yes

Label trnR-UCU-trnT-GGU

Feature Metadata

label_name: trnR-UCU-trnT-GGU
feature_type: igs
region: LSC
length_bp: 1188
variable_sites: 1
pi: 0.00085
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 56.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-trnT-GGU_p1	AGAAGACCTCTGTCCTATCCA	ATTTGAACCGAGGGCTTACG	47.6	50.0	56.9	58.3	1277-1288 bp	1.0	62.2
trnR-UCU-trnT-GGU_p2	AGGTTTAGAAGACCTCTGTCCT	ATTTGAACCGAGGGCTTACG	45.5	50.0	57.9	58.3	1283-1294 bp	1.0	61.5
trnR-UCU-trnT-GGU_p3	AGAAGACCTCTGTCCTATCCA	GGATTTGAACCGAGGGCTTA	47.6	50.0	56.9	57.6	1279-1290 bp	1.0	61.0
trnR-UCU-trnT-GGU_p4	AGGTTTAGAAGACCTCTGTCCT	GGATTTGAACCGAGGGCTTA	45.5	50.0	57.9	57.6	1285-1296 bp	1.0	60.4
trnR-UCU-trnT-GGU_p5	AGAAGACCTCTGTCCTATCCA	ACGCCTTACCATGGGATTAC	47.6	50.0	56.9	57.4	1260-1271 bp	1.0	59.3