Hotspot

trnC-GCA-petN

LSC · 9545-10361 · 817 bp

Back to Hyoseris

Pi 0.00122

MarkerSeek score 59.50

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 817
variable_sites: 1
pi: 0.00122
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTCCCCAGTTCAAATCCGG	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.1	58.2	874 bp	1.0	78.2
trnC-GCA-petN_p2	GGGGGACTGCAAATCCTTTT	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.4	58.2	892 bp	1.0	76.0
trnC-GCA-petN_p3	TTTCCCCAGTTCAAATCCGG	GCCCAAGCGAGACTTACTAT	50.0	50.0	58.1	57.1	873 bp	1.0	72.3
trnC-GCA-petN_p4	TTTCCCCAGTTCAAATCCGG	ATTAAAGCAGCCCAAGCGAG	50.0	50.0	58.1	58.9	882 bp	1.0	72.2
trnC-GCA-petN_p5	ATTGTGTATCGTTTTGGCGG	AGCCCAAGCGAGACTTACTA	45.0	50.0	57.1	58.2	931 bp	1.0	71.6