Hotspot

matK-trnK-UUU

LSC · 3657-4390 · 734 bp

Back to Hyoseris

Pi 0.00136

MarkerSeek score 59.70

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 734
variable_sites: 1
pi: 0.00136
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	ACCATGATCATGAGCAAGTACA	AACGGTAGAGTACTCGGCTT	40.9	50.0	57.5	58.2	865-866 bp	1.0	58.9
matK-trnK-UUU_p2	ACCATGATCATGAGCAAGTACA	ACGGTAGAGTACTCGGCTTT	40.9	50.0	57.5	58.2	864-865 bp	1.0	58.9
matK-trnK-UUU_p3	ACCATGATCATGAGCAAGTACA	TTGGGTTGCTAACTCAACGG	40.9	50.0	57.5	58.4	880-881 bp	1.0	57.0
matK-trnK-UUU_p4	ACCATGATCATGAGCAAGTACA	GGGTTGCTAACTCAACGGTA	40.9	50.0	57.5	57.5	878-879 bp	1.0	56.6
matK-trnK-UUU_p5	AACCATGATCATGAGCAAGT	ACGGTAGAGTACTCGGCTTT	40.0	50.0	55.0	58.2	865-866 bp	1.0	54.9