Hotspot

rpl32

SSC · 127305-127472 · 168 bp

Back to Hyoscyamus

Pi 0.457

MarkerSeek score 76.00

Primer available yes

Label rpl32

Feature Metadata

label_name: rpl32
feature_type: gene
region: SSC
length_bp: 168
variable_sites: 74
pi: 0.457
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 76.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32_p1	ACTCCCCACCTTGGAATAGA	CTCCTTTGAAATAGGCCCGT	50.0	50.0	57.3	57.6	953 bp	0.5	51.4
rpl32_p2	ACTCCCCACCTTGGAATAGA	TGAGCAGTGAATCAAACGGG	50.0	50.0	57.3	58.5	919 bp	0.5	50.9
rpl32_p3	ACTCCCCACCTTGGAATAGA	GCAGTGAATCAAACGGGGAT	50.0	50.0	57.3	58.5	916 bp	0.5	50.4
rpl32_p4	ACTCCCCACCTTGGAATAGA	TCTCCTTTGAAATAGGCCCG	50.0	50.0	57.3	57.3	954 bp	0.5	49.0
rpl32_p5	ACTCCCCACCTTGGAATAGAA	CTCCTTTGAAATAGGCCCGT	47.6	50.0	58.1	57.6	953 bp	0.5	48.1