Hotspot

rpl32-ndhF

SSC · 127473-128238 · 766 bp

Back to Hyoscyamus

Pi 0.443

MarkerSeek score 80.70

Primer available yes

Label rpl32-ndhF

Feature Metadata

label_name: rpl32-ndhF
feature_type: igs
region: SSC
length_bp: 766
variable_sites: 288
pi: 0.443
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.85
markerseek_score: 80.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-ndhF_p1	ACTCCCCACCTTGGAATAGA	AACGGGTCGTGTAAACCAAA	50.0	45.0	57.3	58.0	1693 bp	0.5	54.4
rpl32-ndhF_p2	ACTCCCCACCTTGGAATAGA	ACGGGTCGTGTAAACCAAAA	50.0	45.0	57.3	58.0	1692 bp	0.5	54.4
rpl32-ndhF_p3	ACTCCCCACCTTGGAATAGA	TGGGTCGATCAAGTATCCGA	50.0	50.0	57.3	57.9	1456 bp	0.5	54.2
rpl32-ndhF_p4	ACTCCCCACCTTGGAATAGA	TCTGAAAAGCCCACATACGG	50.0	50.0	57.3	57.9	1313 bp	0.5	53.8
rpl32-ndhF_p5	ACTCCCCACCTTGGAATAGA	GTGGGTCGATCAAGTATCCG	50.0	55.0	57.3	57.9	1457 bp	0.5	53.6