Hotspot

ndhD-ccsA

SSC · 125069-125299 · 231 bp

Back to Hyoscyamus

Pi 0.514

MarkerSeek score 76.50

Primer available yes

Label ndhD-ccsA

Feature Metadata

label_name: ndhD-ccsA
feature_type: igs
region: SSC
length_bp: 231
variable_sites: 109
pi: 0.514
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 76.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-ccsA_p1	TATCTTGACGAAATGGGCGG	ACCCAAGGCTTTGCTACTTC	50.0	50.0	58.1	58.1	1275 bp	0.5	58.9
ndhD-ccsA_p2	TGACGAAATGGGCGGAATAG	ACCCAAGGCTTTGCTACTTC	50.0	50.0	58.1	58.1	1270 bp	0.5	58.9
ndhD-ccsA_p3	TATCTTGACGAAATGGGCGG	CCCAAGGCTTTGCTACTTCA	50.0	50.0	58.1	58.1	1274 bp	0.5	58.9
ndhD-ccsA_p4	TGACGAAATGGGCGGAATAG	CCCAAGGCTTTGCTACTTCA	50.0	50.0	58.1	58.1	1269 bp	0.5	58.9
ndhD-ccsA_p5	TGACGAAATGGGCGGAATAG	GCTCTTCAATCCGAGTGGTT	50.0	50.0	58.1	57.9	1207 bp	0.5	58.8