Hotspot

ndhE-psaC

SSC · 122951-123201 · 251 bp

Back to Hyoscyamus

Pi 0.431

MarkerSeek score 76.90

Primer available yes

Label ndhE-psaC

Feature Metadata

label_name: ndhE-psaC
feature_type: igs
region: SSC
length_bp: 251
variable_sites: 93
pi: 0.431
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 76.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE-psaC_p1	AGGTGCAATTGCTGTAGCTC	CGTGGAGCAGAAGCAATTTG	50.0	50.0	58.5	58.0	875 bp	0.5	55.4
ndhE-psaC_p2	AGGTGCAATTGCTGTAGCTC	AAGCCGCTAAAGTCGCTAAA	50.0	45.0	58.5	57.9	1421 bp	0.5	54.9
ndhE-psaC_p3	AGGTGCAATTGCTGTAGCTC	TCGTGGAGCAGAAGCAATTT	50.0	45.0	58.5	58.1	876 bp	0.5	54.8
ndhE-psaC_p4	AGGTGCAATTGCTGTAGCTC	TTCGTGGAGCAGAAGCAATT	50.0	45.0	58.5	58.1	877 bp	0.5	54.8
ndhE-psaC_p5	AGGTGCAATTGCTGTAGCTC	GAGCACAGGTTTGTCGGTAA	50.0	50.0	58.5	58.1	1050 bp	0.5	54.6