Hotspot

ndhG-ndhE

SSC · 122486-122644 · 159 bp

Back to Hyoscyamus

Pi 0.466

MarkerSeek score 76.50

Primer available yes

Label ndhG-ndhE

Feature Metadata

label_name: ndhG-ndhE
feature_type: igs
region: SSC
length_bp: 159
variable_sites: 68
pi: 0.466
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 76.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhE_p1	CTATTTTGTAGCTGCTGCGC	CGTGGAGCAGAAGCAATTTG	50.0	50.0	57.9	58.0	1214 bp	0.5	59.0
ndhG-ndhE_p2	CTATTTTGTAGCTGCTGCGC	TCGTGGAGCAGAAGCAATTT	50.0	45.0	57.9	58.1	1215 bp	0.5	58.4
ndhG-ndhE_p3	CTATTTTGTAGCTGCTGCGC	TTCGTGGAGCAGAAGCAATT	50.0	45.0	57.9	58.1	1216 bp	0.5	58.4
ndhG-ndhE_p4	CTATTTTGTAGCTGCTGCGC	AGAAATCAGTTGGACAGGCG	50.0	50.0	57.9	58.2	1270 bp	0.5	57.7
ndhG-ndhE_p5	GCCTTTTCCTTGGGATTGGT	CGTGGAGCAGAAGCAATTTG	50.0	50.0	58.4	58.0	1273 bp	0.5	56.9