Hotspot

ycf1-rps15

SSC · 116777-117172 · 396 bp

Back to Hyoscyamus

Pi 0.425

MarkerSeek score 76.30

Primer available yes

Label ycf1-rps15

Feature Metadata

label_name: ycf1-rps15
feature_type: igs
region: SSC
length_bp: 396
variable_sites: 142
pi: 0.425
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.84
markerseek_score: 76.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-rps15_p1	TGAATTGGAATGGCGTCGAT	AGTCGTTGACGTTTTCCCAA	45.0	45.0	58.0	58.0	829 bp	0.5	59.5
ycf1-rps15_p2	TGAATTGGAATGGCGTCGAT	GCCTAATTGTTCGGGTCCAT	45.0	50.0	58.0	57.9	1303 bp	0.5	59.3
ycf1-rps15_p3	TGAATTGGAATGGCGTCGAT	ATGAGTCGAAGAACACCGTG	45.0	50.0	58.0	57.9	1119 bp	0.5	59.2
ycf1-rps15_p4	TGAATTGGAATGGCGTCGAT	CCAACGTGTTACATAGGGCA	45.0	50.0	58.0	57.9	1243 bp	0.5	58.9
ycf1-rps15_p5	TGAATTGGAATGGCGTCGAT	AGCTATCCGACTCAACTCCA	45.0	50.0	58.0	57.9	1369 bp	0.5	58.5