Hotspot

ycf1

SSC · 126474-131840 · 5367 bp

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Pi 0.00242

MarkerSeek score 53.90

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5367
variable_sites: 13
pi: 0.00242
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCGGTTCGACAATCAATATTCCT	ACAATTACGCGAGATCAGACA	39.1	42.9	58.0	57.5	306 bp	1.0	45.1
ycf1_p2	TCGGTTCGACAATCAATATTCC	ACAATTACGCGAGATCAGACA	40.9	42.9	56.6	57.5	306 bp	1.0	42.1
ycf1_p3	CGGTTCGACAATCAATATTCCT	ACAATTACGCGAGATCAGACA	40.9	42.9	56.6	57.5	305 bp	1.0	42.0
ycf1_p4	TCGGTTCGACAATCAATATTCCT	ACAATTACGCGAGATCAGACAT	39.1	40.9	58.0	58.0	306 bp	1.0	41.3
ycf1_p5	TCGGTTCGACAATCAATATTCCT	GACAATTACGCGAGATCAGAC	39.1	47.6	58.0	56.9	307 bp	1.0	41.3