Hotspot

rpoC1

LSC · 21532-24339 · 2808 bp

Back to Hyophorbe

Pi 0.00142

MarkerSeek score 56.80

Primer available yes

Label rpoC1

Feature Metadata

label_name: rpoC1
feature_type: gene
region: LSC
length_bp: 2808
variable_sites: 4
pi: 0.00142
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC1_p1	GAGAGACCCGAATCCCTACA	TTGCTCGTTCGAGAACTACG	55.0	50.0	57.9	58.0	2979-2987 bp	1.0	79.2
rpoC1_p2	AGAGACCCGAATCCCTACAG	TTGCTCGTTCGAGAACTACG	55.0	50.0	57.9	58.0	2978-2986 bp	1.0	79.2
rpoC1_p3	TGAAACTGGAGAGACCCGAA	TTGCTCGTTCGAGAACTACG	50.0	50.0	58.3	58.0	2987-2995 bp	1.0	77.5
rpoC1_p4	GAGAGACCCGAATCCCTACA	GCTCGTTCGAGAACTACGAT	55.0	50.0	57.9	57.5	2977-2985 bp	1.0	75.9
rpoC1_p5	AGAGACCCGAATCCCTACAG	GCTCGTTCGAGAACTACGAT	55.0	50.0	57.9	57.5	2976-2984 bp	1.0	75.9