Hotspot

ndhD

SSC · 117790-119202 · 1413 bp

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Pi 0

MarkerSeek score 35.00

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1413
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 35.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	TGCATTGTACAGCGAACGAT	ATTCGCGGGTTCCTCAATTT	45.0	45.0	58.3	58.2	1540 bp	1.0	76.4
ndhD_p2	TTTGCATTGTACAGCGAACG	ATTCGCGGGTTCCTCAATTT	45.0	45.0	57.7	58.2	1542 bp	1.0	76.2
ndhD_p3	TGCATTGTACAGCGAACGAT	GATATTCGCGGGTTCCTCAA	45.0	50.0	58.3	57.8	1543 bp	1.0	75.9
ndhD_p4	TTTGCATTGTACAGCGAACG	GATATTCGCGGGTTCCTCAA	45.0	50.0	57.7	57.8	1545 bp	1.0	75.7
ndhD_p5	TTGCATTGTACAGCGAACGA	ATTCGCGGGTTCCTCAATTT	45.0	45.0	58.5	58.2	1541 bp	1.0	74.7