Hotspot

atpF

LSC · 12278-13635 · 1358 bp

Back to Hyophorbe

Pi 0.00295

MarkerSeek score 60.60

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1358
variable_sites: 4
pi: 0.00295
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	ACTAATTTCGTCGGCTCGAA	AGAACTCTGTTCTTTGTTAGTCCT	45.0	37.5	57.4	57.5	1512-1517 bp	1.0	40.5
atpF_p2	ACTAATTTCGTCGGCTCGAA	AGTCCTATCTATAAGAGGAGAGCA	45.0	41.7	57.4	57.3	1494-1499 bp	1.0	40.5
atpF_p3	GGCTCGAAGGGTTACCATTA	AGAACTCTGTTCTTTGTTAGTCCT	50.0	37.5	57.0	57.5	1500-1505 bp	1.0	40.5
atpF_p4	ACTAATTTCGTCGGCTCGAA	AGAACTCTGTTCTTTGTTAGTCC	45.0	39.1	57.4	56.2	1512-1517 bp	1.0	40.5
atpF_p5	ACTAATTTCGTCGGCTCGAA	GAACTCTGTTCTTTGTTAGTCCT	45.0	39.1	57.4	56.2	1511-1516 bp	1.0	40.5