Hotspot

rpl22-rps19

IRb · 85830-85882 · 53 bp

Back to Hyophorbe

Pi 0.0208

MarkerSeek score 67.80

Primer available yes

Label rpl22-rps19

Feature Metadata

label_name: rpl22-rps19
feature_type: igs
region: IRb
length_bp: 53
variable_sites: 1
pi: 0.0208
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 67.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22-rps19_p1	CGTCCGCGAATCTGATCAAT	TCTGACTTTCGCGAGACATG	50.0	50.0	58.5	58.0	218 bp	1.0	78.0
rpl22-rps19_p2	CGTCCGCGAATCTGATCAAT	CCTACTCTGACTTTCGCGAG	50.0	55.0	58.5	57.8	223 bp	1.0	76.6
rpl22-rps19_p3	CGTCCGCGAATCTGATCAAT	CTCTGACTTTCGCGAGACAT	50.0	50.0	58.5	57.7	219 bp	1.0	76.0
rpl22-rps19_p4	CGTCCGCGAATCTGATCAAT	CAAATTGGGAGAATTCGCGC	50.0	50.0	58.5	58.5	242 bp	1.0	74.3
rpl22-rps19_p5	CGTCCGCGAATCTGATCAAT	TTCGCGAGACATGCAAGAAA	50.0	45.0	58.5	58.5	211 bp	1.0	74.3