Hotspot

ycf1

SSC · 131304-137051 · 5748 bp

Back to Hymenopus

Pi 0.00645

MarkerSeek score 56.50

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5748
variable_sites: 37
pi: 0.00645
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TGCTTTGATAATAGGTCTGCTTCA	CCAAGGGCTATTTCATCCCG	37.5	55.0	58.3	58.4	273 bp	1.0	44.0
ycf1_p2	TGCTTTGATAATAGGTCTGCT	CCAAGGGCTATTTCATCCCG	38.1	55.0	54.7	58.4	273 bp	1.0	43.7
ycf1_p3	GCTTTGATAATAGGTCTGCTTCA	CCAAGGGCTATTTCATCCCG	39.1	55.0	56.7	58.4	272 bp	1.0	43.4
ycf1_p4	TGCTTTGATAATAGGTCTGCTTC	CCAAGGGCTATTTCATCCCG	39.1	55.0	56.7	58.4	273 bp	1.0	43.4
ycf1_p5	TGCTTTGATAATAGGTCTGCTTCA	TTGGGACCAAGGGCTATTTC	37.5	50.0	58.3	57.5	279 bp	1.0	42.9