Hotspot

ndhG-ndhI

SSC · 125866-126328 · 463 bp

Back to Hymenopus

Pi 0.00907

MarkerSeek score 65.70

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 463
variable_sites: 4
pi: 0.00907
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 65.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	TCCCAACGAAAAGGCAGAAT	TGCTTTAGGGCGTTTACCAA	45.0	45.0	57.7	57.7	658-680 bp	1.0	78.6
ndhG-ndhI_p2	TCCCAACGAAAAGGCAGAAT	TTGCTTTAGGGCGTTTACCA	45.0	45.0	57.7	57.7	659-681 bp	1.0	78.6
ndhG-ndhI_p3	TCCCAACGAAAAGGCAGAAT	AGGGCGTTTACCAATGTCAA	45.0	45.0	57.7	57.7	652-674 bp	1.0	78.6
ndhG-ndhI_p4	ATTGGTCCAGGCAAATCCAT	TGCTTTAGGGCGTTTACCAA	45.0	45.0	57.4	57.7	558-580 bp	1.0	77.0
ndhG-ndhI_p5	ATTGGTCCAGGCAAATCCAT	TTGCTTTAGGGCGTTTACCA	45.0	45.0	57.4	57.7	559-581 bp	1.0	77.0