Hotspot

ndhF-rpl32

SSC · 118499-119607 · 1109 bp

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Pi 0.0113

MarkerSeek score 69.60

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1109
variable_sites: 12
pi: 0.0113
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 69.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	CTGCAAGTGTAACGAAGGGT	AAAAAGCTTTCAACGCGGTC	50.0	45.0	58.1	58.2	1241-1258 bp	1.0	81.7
ndhF-rpl32_p2	CTGCAAGTGTAACGAAGGGT	GAAAAAGCTTTCAACGCGGT	50.0	45.0	58.1	58.2	1242-1259 bp	1.0	81.7
ndhF-rpl32_p3	ACGAAGGGTATGATCCACGA	AAAAAGCTTTCAACGCGGTC	50.0	45.0	58.2	58.2	1230-1247 bp	1.0	80.9
ndhF-rpl32_p4	ACGAAGGGTATGATCCACGA	GAAAAAGCTTTCAACGCGGT	50.0	45.0	58.2	58.2	1231-1248 bp	1.0	80.9
ndhF-rpl32_p5	CTGCAAGTGTAACGAAGGGT	TTCAACGCGGTCCAATATCC	50.0	50.0	58.1	58.3	1233-1250 bp	1.0	80.3