Hotspot

rpl33-rps18

LSC · 72044-72341 · 298 bp

Back to Hymenopus

Pi 0.0341

MarkerSeek score 63.00

Primer available yes

Label rpl33-rps18

Feature Metadata

label_name: rpl33-rps18
feature_type: igs
region: LSC
length_bp: 298
variable_sites: 9
pi: 0.0341
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33-rps18_p1	CCAAGCATACAATTCATGGGG	GGGGTCGCTTGGACTTAAAA	47.6	50.0	57.3	58.1	361-376 bp	1.0	71.2
rpl33-rps18_p2	CCAAGCATACAATTCATGGGG	TCAATTCGATCCCCCGATTG	47.6	50.0	57.3	57.7	404-419 bp	1.0	69.8
rpl33-rps18_p3	AGCATACAATTCATGGGGAGA	GGGGTCGCTTGGACTTAAAA	42.9	50.0	56.8	58.1	358-373 bp	1.0	67.2
rpl33-rps18_p4	CCAAGCATACAATTCATGGGG	ATTCGATCCCCCGATTGGAT	47.6	50.0	57.3	58.6	401-416 bp	1.0	66.7
rpl33-rps18_p5	AGCATACAATTCATGGGGAGA	TCAATTCGATCCCCCGATTG	42.9	50.0	56.8	57.7	401-416 bp	1.0	65.8